Yeast Nitrogen Base w/o AA, Carbohydrate & w/AS (YNB) (Powder)

Cat no: Y2025

Supplier: United States Biological

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Yeast Nitrogen Base (YNB) media have been prepared according the formulae of Wickerham (1943-51) and Burkholder (1943). YNB is a well defined composition of salts, vitamins, amino acids and a nitrogen source for a vigorous growth of Sacharomyces cerevisiae. Yeast Nitrogen Base without amino acids (YNB w/o AA), Y2025, is used for selecting yeasts based on amino acid and carbohydrate requirements. In Y2025, Ammonium Sulfate is included as a readily available nitrogen source for nitrogen assimilation. The medium includes all other vitamins, mineral salts and trace elements required for a vigorous growth of yeast cells. Compared to full YNB medium, Y2025 lacks Histidine, Methionine and Trypthophan. In combination with

Catalogue number:	Y2025
Size:	250g
Alternative names:	Wickerham formula without amino acids & with ammonium sulfate
References:	US Biological application references: 1. Falcon-Perez, J.M. et al., (1999) J. Biol. Chem. 274:23584-23590. 2. Shell, S.S. et al., (2007) PNAS 104:10956-10961. 3. Reiner, S. et al. (2006) Mol Biol Cell 17:90-103. 4. Cronwright, G.R. et al., (2002) Appl. & Env. Microbiology 68:4448-4456. 1. Difco Manual Tenth Edition, pp.1136-1141(1984). 2. Ronald, M.: Atlas, Handbook of Microbiological Media, Lawrence C. Parks, ed., CRC Press (1993).

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Yeast Nitrogen Base w/o AA, Carbohydrate & w/AS (YNB) (Powder)

Cat no: Y2025

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