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Chromatography

Chromatography
Looking for reliable and cost effective Chromatography products for your research?

Chromatography is a standard technique for analyzing molecules, such as proteins or DNA. In biological laboratories, researchers use various forms of liquid chromatography (LC) or gas chromatography (GC) to separate, detect and/or analyze biomolecules in pharmaceutical, cosmetic, food & drink, agricultural, industrial, and other materials.


In a simplified liquid chromatography setup, the biomolecule to be analyzed, called the analyte, is tagged with a fluorescent molecule and then temporarily bonded to plastic beads packed in a glass or metal column. Next, a liquid solvent, or eluent, is pumped through the column and around the beads, causing the stationary analyte molecules to begin binding to molecules in the moving eluent. As the liquid flows out the end of the column, an optical detector records the number of analyte molecules in it by counting the fluorescent tags as they pass by.

By plotting the concentration of analyte molecules flowing out of the column over time, and comparing the plot to a library of standards, researchers can determine molecular weight distributions, shapes, sizes, chemical reactivities and other characterizations of the biomolecules.

Many different methods and platform configurations are used in chromatography systems: Fast protein liquid chromatography (FPLC), reversed-phase chromatography (RPC), gel permeation chromatography (GPC), ion exchange chromatography, affinity chromatography, and high performance liquid chromatography (HPLC). These use spectrophotomers or mass spectrometers as detectors.

Chromatography accessories and equipment includes autosamplers, beads, liquid and gas solvents, fluorescent tags and dyes, and columns optimized for analyzing and separating specific chemical compounds. 

Browse the product range below or simply use the Search Tool above to help find the best products.

Once you have found a product, complete a ‘Get a Quote’ form and you will get the best price!

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AB SCIEX 4500 Series
AB SCIEX 4500 Series
AB SCIEX

Real-world labs need an industrious LC/MS/MS workhorse for trustworthy results hour after hour, day after day. The new AB SCIEX 4500 Series takes the...

UV Protective Eye and Face Wear
UV Protective Eye and Face Wear
UVMAN

You use UV light right? You protect your eyes and face from exposure to harmful rays don't you....? You don't? Better get yourself over to our website for a...

Spectroline Magifying UV Lamp
Spectroline Magifying UV Lamp
UVMAN

Have you ever thought how great it would be to be able to examine items under UV Lamp with a magnifyer? Now you can have it all with one very handy UV...

Spectroline Transilluminator
Spectroline Transilluminator
UVMAN

Want a quality Transilluminator without the need to take out a second mortgage? Take a look at the Spectroline range we offer and then contact us for a price...

Spectroline CellBlaster - Mobile Phone Sanitiser
Spectroline CellBlaster - Mobile Phone Sanitiser
UVMAN

Cleaned your mobile phone lately? Have you any idea how many bugs and germs are likely to be lurking there? If I were to tell you that it has been...

Spectroline Bench and Display UV Lamps
Spectroline Bench and Display UV Lamps
UVMAN

The Spectroline X-Series range offers considerable flexibiity for those seeking quality fixed UV light sources. Available in all three (Short Wave, Medium...

PURELAB flex - ultrapure water direct from tap water
PURELAB flex - ultrapure water direct from tap water
ELGA LabWater

The ELGA PURELAB flex delivers ultrapure water direct from tap water: saves money no need for feed system, absolute water purity of up to 18.2MΩ-cm,...

PURELAB Option-Q
PURELAB Option-Q
ELGA LabWater

Tap to type I ultrapure water direct from a potable tap water supply.The PURELAB Option-Q is ideal for laboratories who need up to 100 liters of 18.2...

PURELAB Option-R
PURELAB Option-R
ELGA LabWater

The PUREL AB Option provides Type II+ pure water on demand with a wide range of flow-rates to suit your needs.Applications range from glassware washing and...

PURELAB Option-S
PURELAB Option-S
ELGA LabWater

The PURELAB Option-S provides Type II pure water on demand with a wide range of flow-rates to suit your needs.Applications range from glassware washing and...

PURELAB Pulse
PURELAB Pulse
ELGA LabWater

The PURELAB Pulse is the cost-effective choice for laboratories that require higher output volumes especially in hard water areas.Applications range from...

PURELAB Classic
PURELAB Classic
ELGA LabWater

The PURELAB Classic system combines high performance with economy to deliver 18.2 MΩ-cm-1 water at a very cost-effective price.Despite its budget price the...

CENTRA-RDS storage and recirculating distribution system
CENTRA-RDS storage and recirculating distribution system
ELGA LabWater

The CENTRA has revolutionised the way that large volumes of pure water are produced, stored and distributed.Instead of a central lab system, comprising of an...

CENTRA-R 60/120 water purification, storage, control and distribution system
CENTRA-R 60/120 water purification, storage, control and distribution system
ELGA LabWater

The CENTRA has revolutionised the way that pure water is produced, stored and distributed. Instead of a traditionally engineered central lab system,...

Sterisonic Series Cell Culture Incubators
Sterisonic Series Cell Culture Incubators
Panasonic Healthcare Company

Sterisonic Incubators offer industry’s fastest cell culture CO2 incubator decontamination process using hydrogen peroxide vapor (H2O2) for highly...

Get a discount for promotion of our products!
Get a discount for promotion of our products!
Hycult Biotech

Hycult Biotech is always looking for recent articles of importance to Innate Immune research that we can place on our website or in our HycultScope, our...

1-plate assays available!
1-plate assays available!
Hycult Biotech

Hycult Biotech now offers 1-plate assays from a selection of 15 assays in the Hycult Biotech product range.

BSA-free Antibodies for Immuno Assay
BSA-free Antibodies for Immuno Assay
Hycult Biotech

Hycult Biotech now offers antibodies specified for Immuno Assays!

Flow Cytometry Brochure
Flow Cytometry Brochure
Hycult Biotech

This new brochure contains the Hycult Biotech antibodies which are suitable for the application Flow Cytometry.To have a look at the brochure, check our...

LPS Core monoclonal antibody, clone WN1 222-5
LPS Core monoclonal antibody, clone WN1 222-5
Hycult Biotech

The mouse monoclonal antibody clone WN1 222-5 recognizes the core region of lipopolysaccharide (LPS), whereas it lacks reactivity with free lipid A and Rd2...

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