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Flow Cytometry

Flow Cytometry
Looking for reliable and cost effective Flow Cytometric products for your research?

Flow cytometry is used in a wide range of applications, including clinical testing and point-of-care diagnostics, genetics research, agricultural and veterinary sciences, food processing, and water purification testing. It is a fast, laser-based method for detecting pathogens or cancerous cells in a liquid, such as blood serum, and it can also be used to separate individual cells or molecules, for instance DNA amplified by PCR.

The technique of flow cytometry measures the number and type of particles that have been tagged or labeled with fluorescent dye molecules as part of a clinical assay or other lab procedure. A flow cytometer nozzle shoots a focused stream of liquid containing the assayed molecules at the rate of thousands of particles per second across a laser beam of a given wavelength. Each molecule in the liquid passes one-by-one through the beam, scattering the light. At the same time, the fluorescent tags on some of the molecules become energized by the light rays, giving off light at a longer wavelength than the laser beam.

The scattered and excited light rays are captured by electro-optical detectors, which convert the photons into electrical signals. These signals can then be analyzed by a computer to determine the composition of the molecules being tested.

Several different tags or dyes can be multiplexed in flow cytometery, to detect different molecules in the same operation. Some of the dyes can be “reversed,” or chemically removed from tagged molecules, in order to recycle them back into a patient’s blood stream, for example in cell therapy.

Laboratory equipment and supplies for flow cytometry include lasers and optical detectors; clinical and research laboratory assay kits; antibodies, proteins and peptides; antibody specific tags, conjugates (dyes) and dye combinations; staining or isolation reagents; and particle count standards.

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CAP1 peptide YLSGANLNL (HLA-A*0201)
CAP1 peptide YLSGANLNL (HLA-A*0201)
IBA GmbH

CAP1 peptide YLSGANLNL (HLA-A*0201) for stimulation of T-cells. Single peptide (YLSGANLNL) for stimulation of human CAP1-specific CD8+ T-cells. The peptide...

Ovalbumin peptide SIINFEKL (H-2 Kb)
Ovalbumin peptide SIINFEKL (H-2 Kb)
IBA GmbH

Ovalbumin peptide SIINFEKL (H-2 Kb) for stimulation of T cells. Single peptide (SIINFEKL) for stimulation of mouse Ovalbumin-specific CD8+ T cells. The...

CMV pp65 peptide NLVPMVATV (HLA-A*0201)
CMV pp65 peptide NLVPMVATV (HLA-A*0201)
IBA GmbH

CMV pp65 peptide NLVPMVATV (HLA-A0201) for stimulation of T-cells. Single peptide (NLVPMVATV) for stimulation of human CMV-specific CD8+ T-cells. The peptide...

MHC I Streptamer(R) Best CELLer Staining Kit
MHC I Streptamer(R) Best CELLer Staining Kit
IBA GmbH

STREPTAMER(R) Best CELLer fluorescent MHC I Streptamer(R) reagents for detection and isolation of antigen-specific CD8+ T cells by flow cytometry select your...

CD4+ CD25+ CD45RA+ Treg Fab Streptamer(R) Isolation Kit MB, human
CD4+ CD25+ CD45RA+ Treg Fab Streptamer(R) Isolation Kit MB, human
IBA GmbH

For isolation of CD4+ CD25+ CD45RA+ regulatory T cells from peripheral blood mononuclear cells. The kit includes all Fab Streptamer components for triple...

CD3+ CD45RO+ Tcm Fab Streptamer(R) Isolation Kit MB, human
CD3+ CD45RO+ Tcm Fab Streptamer(R) Isolation Kit MB, human
IBA GmbH

For isolation of CD3+ CD45RO+ central memory T cells from peripheral blood mononuclear cells. The kit includes all Fab Streptamer components for double...

StrepMan magnet
StrepMan magnet
IBA GmbH

StrepMan permanent magnet for Streptamer(R) cell isolation with Strep-Tactin(R) Magnetic Microbeads; for 2x15 ml and 2x50 ml Falcon tubes

CD3+ CD62L+ CD45RO+ Tcm Fab Streptamer(R) Isolation Kit MB, human
CD3+ CD62L+ CD45RO+ Tcm Fab Streptamer(R) Isolation Kit MB, human
IBA GmbH

For isolation of CD3+ CD62L+ CD45RO+ central memory T cells from peripheral blood mononuclear cells. The kit includes all Fab Streptamer components for...

CD8+ CD62L+ CD45RA+ naive T cell Fab Streptamer(R) Isolation Kit MB, human
CD8+ CD62L+ CD45RA+ naive T cell Fab Streptamer(R) Isolation Kit MB, human
IBA GmbH

For isolation of CD8+ CD62L+ CD45RA+ naive T cells from peripheral blood mononuclear cells. The kit includes all Fab Streptamer components for a triple...

CD4+ CD25+ Treg Fab Streptamer(R) Isolation Kit MB, human
CD4+ CD25+ Treg Fab Streptamer(R) Isolation Kit MB, human
IBA GmbH

For isolation of CD4+ CD25+ regulatory T cells from peripheral blood mononuclear cells. The kit includes all Fab Streptamer components for double positive...

CD8 Fab Streptamer(R) Isolation Kit MB, human
CD8 Fab Streptamer(R) Isolation Kit MB, human
IBA GmbH

For isolation of CD8+ T cells from peripheral blood mononuclear cells. The kit includes all Fab Streptamer components for positive cell isolation with...

Analyze your cells with more colors at lower prices!
Analyze your cells with more colors at lower prices!
BD (Becton, Dickinson and Company)

New brilliant antibodies, and new lower prices!For flow cytometry reagents in general, "bright is better." The violet-excitable BD Horizon™ BV421 and...

S3™ Cell Sorter
S3™ Cell Sorter
Bio-Rad Laboratories

The S3 cell sorter offers affordable high speed sorting with up to 99% purity. Automated set-up and quality control enable researchers to perform 1-4 colour...

Fluorescent custom oligonucleotides
Fluorescent custom oligonucleotides
IBA GmbH

Excellent service for custom-made fluorescently labeled oligonucleotides. More than 150 labels to choose from Double or multiple labeling possible. Licensed...

IGF1 Antibody
IGF1 Antibody
ABBIOTEC

Insulin-like Growth Factors IGF1 and IGF2 share 76% sequence identity and are 50% related to pro-insulin. They are non-glycosylated, single-chain proteins of...

IL-1 beta Antibody
IL-1 beta Antibody
ABBIOTEC

Interleukin-1 (IL-1) is a cytokine produced by activated macrophages. IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and...

IL-10 Antibody
IL-10 Antibody
ABBIOTEC

Interleukin 10 (IL-10) is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. IL-10 has pleiotropic effects in immunoregulation...

CD3 Antibody
CD3 Antibody
ABBIOTEC

The CD3 antibody recognizes the 17-19 kD ε-chain of CD3 within the CD3 antigen/T cell antigen receptor (TCR) complex. The CD3 antigen is expressed...

CD4 Antibody
CD4 Antibody
ABBIOTEC

CD4 antigen is found on a T-cell subset (helper/inducer) representing 45% of peripheral blood lymphocytes. CD4 is also present on 80% of thymocytes and at a...

CD31 (N-term) Antibody
CD31 (N-term) Antibody
ABBIOTEC

CD31 (PECAM1) is a member of the immunoglobulin (Ig) superfamily that is expressed on the surface of circulating platelets, monocytes, neutrophils, and...

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