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qPCR / Real-Time PCR

qPCR / Real-Time PCR
Looking for reliable and cost effective qPCR / Real-Time PCR products for your research?

Real-time PCR (or qPCR) proceeds in the same way as the standard polymerase chain reaction technique, but the concentration of the target DNA sequence is observed and/or recorded in real time, rather than just at the end of the amplification process. Small amounts of messenger RNA (mRNA), which regulates gene expression in the cell, can be also amplified, once the RNA has been reverse...

...transcribed to complementary DNA (cDNA). Real-time PCR techniques can be used to determine relative gene expression, quantification and/or genotyping of infectious diseases, viruses, GMOs, and pathogens in agriculture, water supplies, and food & beverages.

There are two basic methods of tracking the PCR process in real time using optical detectors. One is to use non-specific fluorescent dyes, which bind with any double-stranded DNA molecule. Another is to use “reporter” probes consisting of an   oligonucleotide complementary to the target sequence at a point between the two primers. The probe contains a linked fluorescent dye molecule and a quencher molecule that inhibits fluorescence. During the polymerization process, the probe specifically binds within the target DNA sequence at the same time as the PCR primers. But once the sequencing action of the polymerase reaches the bound probe, the fluorescent molecule is knocked off, causing an increased fluorescence which can be read. As the sequencing continues along the DNA strand, the probe releases the quencher into solution, and causing a drop in the fluorescence reading. Thus, the technician can “see” and measure the results of each cycle of the DNA amplification as it progresses in real time.

Biochemicals and reagents for qPCR include custom oligonucleotides and gene fragments; pPCR primers and assays; Taq polymerase; probes; real-time PCR kits with different probe chemistries; cell lysing kits; high-throughput fragment library prep solution; transfection reagent; RNA extraction solution; DNA removal enzyme kits; and universal microRNA expression profiling panels and assays.

Instrumentation and equipment for qPCR include real-time PCR detection systems; automated qPCR prep systems; PCR plates and stands; PCR tube strips; heat sealing systems and seals; LIMS systems; single- and multi-channel pipettes; microvolume cuvettes; tissue homogenizers; sample coolers; centrifuges; and nucleic acid analysis systems.

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RealQ Plus 2x Master Mix Green high ROX 4000R
RealQ Plus 2x Master Mix Green high ROX 4000R
B-Bridge International, Inc.

Quantitative PCR has become an important tool for SNP and gene expression analysis. Several different fluorescent chemistries exist for either detection of...

RealQ Plus 2x Master Mix Green high ROX 400R
RealQ Plus 2x Master Mix Green high ROX 400R
B-Bridge International, Inc.

Quantitative PCR has become an important tool for SNP and gene expression analysis. Several different fluorescent chemistries exist for either detection of...

RealQ Plus 2x Master Mix Green low ROX 4000R
RealQ Plus 2x Master Mix Green low ROX 4000R
B-Bridge International, Inc.

Quantitative PCR has become an important tool for SNP and gene expression analysis. Several different fluorescent chemistries exist for either detection of...

RealQ Plus 2x Master Mix Green low ROX 400R
RealQ Plus 2x Master Mix Green low ROX 400R
B-Bridge International, Inc.

Quantitative PCR has become an important tool for SNP and gene expression analysis. Several different fluorescent chemistries exist for either detection of...

RealQ Plus 2x Master Mix Green without ROX 4000R
RealQ Plus 2x Master Mix Green without ROX 4000R
B-Bridge International, Inc.

Quantitative PCR has become an important tool for SNP and gene expression analysis. Several different fluorescent chemistries exist for either detection of...

RealQ Plus 2x Master Mix Green without ROX 400R
RealQ Plus 2x Master Mix Green without ROX 400R
B-Bridge International, Inc.

Quantitative PCR has become an important tool for SNP and gene expression analysis. Several different fluorescent chemistries exist for either detection of...

RealQ Plus 2x Master Mix for Probe high ROX 4000R
RealQ Plus 2x Master Mix for Probe high ROX 4000R
B-Bridge International, Inc.

Quantitative PCR has become an important tool for SNP and gene expression analysis. Several different fluorescent chemistries exist for either detection of...

RealQ Plus 2x Master Mix for Probe high ROX 400R
RealQ Plus 2x Master Mix for Probe high ROX 400R
B-Bridge International, Inc.

Quantitative PCR has become an important tool for SNP and gene expression analysis. Several different fluorescent chemistries exist for either detection of...

RealQ Plus 2x Master Mix for Probe low ROX 4000R
RealQ Plus 2x Master Mix for Probe low ROX 4000R
B-Bridge International, Inc.

Quantitative PCR has become an important tool for SNP and gene expression analysis. Several different fluorescent chemistries exist for either detection of...

RealQ Plus 2x Master Mix for Probe low ROX 400R
RealQ Plus 2x Master Mix for Probe low ROX 400R
B-Bridge International, Inc.

Quantitative PCR has become an important tool for SNP and gene expression analysis. Several different fluorescent chemistries exist for either detection of...

RealQ Plus 2x Master Mix for Probe without ROX 4000R
RealQ Plus 2x Master Mix for Probe without ROX 4000R
B-Bridge International, Inc.

Quantitative PCR has become an important tool for SNP and gene expression analysis. Several different fluorescent chemistries exist for either detection of...

RealQ Plus 2x Master Mix for Probe without ROX 400R
RealQ Plus 2x Master Mix for Probe without ROX 400R
B-Bridge International, Inc.

Quantitative PCR has become an important tool for SNP and gene expression analysis. Several different fluorescent chemistries exist for either detection of...

PIPETMAN M P1000M
PIPETMAN M P1000M
Gilson, Inc.

Pipetman M P1000M, 100-1000µL

PIPETMAN M P200M
PIPETMAN M P200M
Gilson, Inc.

Pipetman M P200M, 20-200µL

PIPETMAN M P20M
PIPETMAN M P20M
Gilson, Inc.

Pipetman M P20M, 2-20µL

PIPETMAN M P10M
PIPETMAN M P10M
Gilson, Inc.

Pipetman M P10M, 0.5-10µL

PIPETMAN L P12x300L
PIPETMAN L P12x300L
Gilson, Inc.

Pipetman L P12x300L, 20-300µL

PIPETMAN L P8x300L
PIPETMAN L P8x300L
Gilson, Inc.

Pipetman L P8x300L, 20-300µL

PIPETMAN L P12x200L
PIPETMAN L P12x200L
Gilson, Inc.

Pipetman L P12x200L, 20-200µL

PIPETMAN L P8x200L
PIPETMAN L P8x200L
Gilson, Inc.

Pipetman L P8x200L, 20-200µL

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