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Protein Expression

Protein Expression
Looking for reliable and cost effective Protein Expression products for your research?

The proteome, or complete set of proteins present in an organism, differs from the genome, which contains all the genetic coding for the organism, because the expression of proteins within a particular cell varies with location in the body, health of the cell, and the organism’s stage of development. Protein expression occurs when messenger RNA (mRNA) uses genetic information...

...transcribed from a cell’s DNA to code the synthesis of (or translate) many identical protein molecules. Once the long polypeptide chain of the protein’s amino acids have been assembled by the mRNA, its components begin to interact chemically, and assisted by enzymes, form bonds which fold up the chain into the molecule’s final 3-dimensional shape. A cell can control protein expression by completely blocking or reducing one or more of the many steps toward synthesis, beginning with RNA transcription.

In the lab, protein expression has been harnessed through a group of techniques to create large quantities of a specific protein molecule for research or therapy purposes. Recombinant DNA can be introduced into a host cell or species, such as a bacteria, virus, yeast, protozoa, or insect or mammalian cell, which then express, or overexpress, the desired proteins. These proteins may be secreted from the host and/or screened and purified using affinity tags. Protein expression is used for medical therapeutics and vaccines, clinical diagnostics, drug discovery and development, genetics and genomics, food & beverage testing, and many other applications.

Instrumentation and equipment for protein expression include blot PVDF membranes; microplate readers; gel electrophoresis imaging systems; microscopy systems; spectrophotometers; cell culture incubators; culture media; cell growth containers; particle analyzers; liquid, ion and HPLC chromatography systems; LC column heaters and chillers; autosamplers; microvolume cuvettes; homogenizers; centrifuges; ultralow freezers; water bath shakers; autoclaves; benchtop transfection systems; roller bottle processing systems; laboratory and sample information management systems; sequencing software; and DNA sequencing services.

Biochemicals and reagents for protein expression include transfection reagents; protein standards; monoclonal antibodies; oligonucleotides; recombinant proteins; vaccines; antibody drug conjugates; PCR primers; gene fragments; ELISA kits and assays; protein extraction kits; purification kits; RNA polymerase; transcription kits; cDNA synthesis kits; stabilizers; endotoxin removal kits and services; bacteria capture kits; custom recombinant protein production; and cell lysing kits.

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CAP1 peptide YLSGANLNL (HLA-A*0201)
CAP1 peptide YLSGANLNL (HLA-A*0201)
IBA GmbH

CAP1 peptide YLSGANLNL (HLA-A*0201) for stimulation of T-cells. Single peptide (YLSGANLNL) for stimulation of human CAP1-specific CD8+ T-cells. The peptide...

Ovalbumin peptide SIINFEKL (H-2 Kb)
Ovalbumin peptide SIINFEKL (H-2 Kb)
IBA GmbH

Ovalbumin peptide SIINFEKL (H-2 Kb) for stimulation of T cells. Single peptide (SIINFEKL) for stimulation of mouse Ovalbumin-specific CD8+ T cells. The...

CMV pp65 peptide NLVPMVATV (HLA-A*0201)
CMV pp65 peptide NLVPMVATV (HLA-A*0201)
IBA GmbH

CMV pp65 peptide NLVPMVATV (HLA-A0201) for stimulation of T-cells. Single peptide (NLVPMVATV) for stimulation of human CMV-specific CD8+ T-cells. The peptide...

MHC I Streptamer(R) Best CELLer Staining Kit
MHC I Streptamer(R) Best CELLer Staining Kit
IBA GmbH

STREPTAMER(R) Best CELLer fluorescent MHC I Streptamer(R) reagents for detection and isolation of antigen-specific CD8+ T cells by flow cytometry select your...

CD4+ CD25+ CD45RA+ Treg Fab Streptamer(R) Isolation Kit MB, human
CD4+ CD25+ CD45RA+ Treg Fab Streptamer(R) Isolation Kit MB, human
IBA GmbH

For isolation of CD4+ CD25+ CD45RA+ regulatory T cells from peripheral blood mononuclear cells. The kit includes all Fab Streptamer components for triple...

CD3+ CD45RO+ Tcm Fab Streptamer(R) Isolation Kit MB, human
CD3+ CD45RO+ Tcm Fab Streptamer(R) Isolation Kit MB, human
IBA GmbH

For isolation of CD3+ CD45RO+ central memory T cells from peripheral blood mononuclear cells. The kit includes all Fab Streptamer components for double...

StrepMan magnet
StrepMan magnet
IBA GmbH

StrepMan permanent magnet for Streptamer(R) cell isolation with Strep-Tactin(R) Magnetic Microbeads; for 2x15 ml and 2x50 ml Falcon tubes

CD3+ CD62L+ CD45RO+ Tcm Fab Streptamer(R) Isolation Kit MB, human
CD3+ CD62L+ CD45RO+ Tcm Fab Streptamer(R) Isolation Kit MB, human
IBA GmbH

For isolation of CD3+ CD62L+ CD45RO+ central memory T cells from peripheral blood mononuclear cells. The kit includes all Fab Streptamer components for...

CD8+ CD62L+ CD45RA+ naive T cell Fab Streptamer(R) Isolation Kit MB, human
CD8+ CD62L+ CD45RA+ naive T cell Fab Streptamer(R) Isolation Kit MB, human
IBA GmbH

For isolation of CD8+ CD62L+ CD45RA+ naive T cells from peripheral blood mononuclear cells. The kit includes all Fab Streptamer components for a triple...

CD4+ CD25+ Treg Fab Streptamer(R) Isolation Kit MB, human
CD4+ CD25+ Treg Fab Streptamer(R) Isolation Kit MB, human
IBA GmbH

For isolation of CD4+ CD25+ regulatory T cells from peripheral blood mononuclear cells. The kit includes all Fab Streptamer components for double positive...

CD8 Fab Streptamer(R) Isolation Kit MB, human
CD8 Fab Streptamer(R) Isolation Kit MB, human
IBA GmbH

For isolation of CD8+ T cells from peripheral blood mononuclear cells. The kit includes all Fab Streptamer components for positive cell isolation with...

StrepMAB-Classic, HRP conjugate
StrepMAB-Classic, HRP conjugate
IBA GmbH

StrepMAB-Classic (Strep-tag(R) II specific monoclonal antibody) conjugated to horseradish peroxidase. For the detection of N- or C-terminal or internal...

Buffer BX; Biotin Elution Buffer
Buffer BX; Biotin Elution Buffer
IBA GmbH

Biotin Elution Buffer BX comes with a high Biotin concentration for competitive displacement of purified recombinant Strep-tag(R) or Twin-Strep-tag(R) fusion...

MagStrep "type3" XT beads
MagStrep "type3" XT beads
IBA GmbH

MagStrep "type3" XT Beads (Strep-Tactin XT coated magnetic beads, 5 % (v/v) suspension) The beads have a high binding capacity combined with very low...

Strep-Tactin(R) Superflow(R) high capacity cartridge
Strep-Tactin(R) Superflow(R) high capacity cartridge
IBA GmbH

Strep-Tactin(R) Superflow(R) high capacity cartridge for one-step purification of recombinant Strep-tag(R) proteins with HPLC or FPLC. The cartridges are...

Gravity flow Strep-Tactin Superflow high cap. col.
Gravity flow Strep-Tactin Superflow high cap. col.
IBA GmbH

Gravity flowStrep-Tactin(R) Superflow(R) high capacity column for one-step purification of recombinant Strep-tag(R) proteins. The Strep-Tactin(R)...

Strep-tag(R) protein purification buffer set
Strep-tag(R) protein purification buffer set
IBA GmbH

Strep-tag(R) Protein Purification Buffer Set; convenient buffer set for the purification of recombinant Strep-tag(R) fusion proteins on immobilized...

His-STREPPER Trial Kit
His-STREPPER Trial Kit
IBA GmbH

The His-STREPPER is an adapter molecule comprising Strep-tagII (SA-WSHPQFEK) conjugated with a nickel charged tris-NTA. It tightly binds the 6xHis-tag and...

Strep/6xHistidine Starter Kit
Strep/6xHistidine Starter Kit
IBA GmbH

Strep/ 6xHistidine Starter Kit H-PR for purification of double tag proteins. This unique Starter Kit contains all reagents essential for the native...

Strep-Tactin(R) Spin Column Kit
Strep-Tactin(R) Spin Column Kit
IBA GmbH

The Strep-Tactin(R) Spin Column Kit allows rapid one-step purification of up to 150 micro g Strep-tag(R) proteins under physiological conditions in less than...

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