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Cloning

Cloning
Looking for reliable and cost effective Cloning products for your research?

Cloning, or copying DNA sequences and inserting them into a vector, such as a plasmid or E. coli bacteria, in order to transfect that DNA into a cell or organism, plays an important role in many types of biological laboratory research. Cloning individual genes or other DNA fragments is a much less daunting process than reproductive cloning of an entire multicellular organism, such as a sheep; it is...

...used on a regular basis in clinical and research labs for synthesizing genes, amplifying DNA, and testing new therapies for genetic disorders.

Laboratory materials for genetic cloning include species-specific PCR primers for RT-PCR (reverse transcription followed by PCR) analysis of mRNA expression; tissue panels and matched pairs of tissue specific or universal cDNA (complementary DNA) from human adult normal and diseased tissues, human fetal tissues, rat, mouse, chicken, canine, monkey and plant tissues; species-specific or tissue-specific probes or oligonucleotides (DNA fragments); amplification, assay and RNA synthesis kits; ligands; cell culture media and albumin; transfection reagents; and primers and adapters.

Equipment for cloning research includes thermal cyclers for high-speed PCR; cooling blocks for incubating samples; hybridization incubators; cryogenic and ultralow freezers; microplate readers; autoclaves; centrifuges; homogenizers; water bath shakers; and DNA and RNA extractors/purifiers.

Additional cloning research aids include DNA and protein sequence analysis software; LIMS software; clone injection services; DNA sequencing services; and strains of E. coli for cloning or subcloning.

Browse the product range below or simply use the Search Tool above to help find the best products.

Once you have found a product, complete a ‘Get a Quote’ form and you will get the best price!

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Enhanced Flp Expression Plasmid:710-FLPe;km
Enhanced Flp Expression Plasmid:710-FLPe;km
B-Bridge International, Inc.

Gene Bridges FLPe expression plasmids enable FLP-mediated site-directed recombination in E. coli, and are propagated at 30C in low copy number. The flpe gene...

Enhanced Flp Expression Plasmid:709-FLPe;amp
Enhanced Flp Expression Plasmid:709-FLPe;amp
B-Bridge International, Inc.

Gene Bridges FLPe expression plasmids enable FLP-mediated site-directed recombination in E. coli, and are propagated at 30C in low copy number. The flpe gene...

iCre-FRT-neo-FRT (codon improved Cre(iCre) with attached FRT flanked, Pro- and Eukaryotic Neomycin selection cassette
iCre-FRT-neo-FRT (codon improved Cre(iCre) with attached FRT flanked, Pro- and Eukaryotic Neomycin selection cassette
B-Bridge International, Inc.

iCre-FRT-neo-FRT PCR template is designed to facilitate the insertion of such a functional cassette into targeting constructs by Red/ET recombination. A...

Recombineering-proficient E.coli strain GB08-red
Recombineering-proficient E.coli strain GB08-red
B-Bridge International, Inc.

E. coli strain GB08-red enables recombineering of plasmids and BACs without the necessity to maintain an expression plasmid in the cells since the necessary...

Direct-cloning-proficient E.coli strain GB05-dir
Direct-cloning-proficient E.coli strain GB05-dir
B-Bridge International, Inc.

Full-length Rac prophage protein RecE and its partner RecT mediate highly efficient linear-to-linear homologous recombination mechanistically distinct from...

Quick & Easy Red/ET E. coli Gene Deletion Kit (license required to purchase)
Quick & Easy Red/ET E. coli Gene Deletion Kit (license required to purchase)
B-Bridge International, Inc.

For fast knockouts or modifications of genes on the E.coli chromosome. Red/ET recombination allows the exchange of genetic information in a base pair...

Quick & Easy Red/ET Conditional Knock Out Kit - loxP (license required to purchase)
Quick & Easy Red/ET Conditional Knock Out Kit - loxP (license required to purchase)
B-Bridge International, Inc.

For fast and simple integration of loxP-sites into large vector plasmids at any intended position (for generation of transgenic mouse models). The included...

Quick & Easy Red/ET Conditional Knock Out Kit - FRT (license required to purchase)
Quick & Easy Red/ET Conditional Knock Out Kit - FRT (license required to purchase)
B-Bridge International, Inc.

For fast and simple integration of FRT-sites into large vector plasmids at any intended position (for generation of transgenic mouse models). The...

Red/ET BAC Subcloning Kit  (license required to purchase)
Red/ET BAC Subcloning Kit (license required to purchase)
B-Bridge International, Inc.

For subcloning of DNA fragments from BACs and cosmids using the Red/ET Recombination System. No restriction sites are necessary and fragments up to 20 kb can...

Red/ET Counter Selection BAC Modification Kit (license required to purchase)
Red/ET Counter Selection BAC Modification Kit (license required to purchase)
B-Bridge International, Inc.

This is a new version of counter-selection cassette pRpsL-neo for the Red/ET Recombination system. It is based on Streptomycin selection. The kit is designed...

Red/ET Quick & Easy BAC Modification Kit (license required to purchase)
Red/ET Quick & Easy BAC Modification Kit (license required to purchase)
B-Bridge International, Inc.

This kit is designed to modify BACs with the revolutionary Red/ET Recombination technology - a powerful tool for introducing modifications such as insertions...

Red/ET Enhanced Eukaryotic FLP Expression Plasmid: pCAGGS-FLPe (Industry price, requires MTA)
Red/ET Enhanced Eukaryotic FLP Expression Plasmid: pCAGGS-FLPe (Industry price, requires MTA)
B-Bridge International, Inc.

pCAGGS-FLPe-IRESpuro is an improved vector for expression of an enhanced FLP recombinase in mouse ES cells and oocytes. FLPe shows an enhanced thermo...

Red/ET Enhanced Eukaryotic FLP Expression Plasmid: pCAGGS-FLPe (Academic price, requires MTA)
Red/ET Enhanced Eukaryotic FLP Expression Plasmid: pCAGGS-FLPe (Academic price, requires MTA)
B-Bridge International, Inc.

pCAGGS-FLPe-IRESpuro is an improved vector for expression of an enhanced FLP recombinase in mouse ES cells and oocytes. FLPe shows an enhanced thermo...

Red/ET Cre Expression Plasmid:706-Cre, tet-selection
Red/ET Cre Expression Plasmid:706-Cre, tet-selection
B-Bridge International, Inc.

The 706-Cre plasmid is designed for use in Cre-mediated genomic manipulations. The plasmid has a pSC101 origin which maintains low copy and replicates at...

Red/ET Cre Expression Plasmid:705-Cre, cm-selection
Red/ET Cre Expression Plasmid:705-Cre, cm-selection
B-Bridge International, Inc.

The 705-Cre plasmid is designed for use in Cre-mediated genomic manipulations. The plasmid has a pSC101 origin which maintains low copy and replicates at...

Red/ET Enhanced Flp Expression Plasmid:708-FLPe, cm-selection
Red/ET Enhanced Flp Expression Plasmid:708-FLPe, cm-selection
B-Bridge International, Inc.

The expression plasmid enables FLP-mediated site specific recombination. The plasmid confer tetracycline (707-FLPe) and propagates at 30Degree C in low copy...

Red/ET Enhanced Flp Expression Plasmid:707-FLPe, tet-selection
Red/ET Enhanced Flp Expression Plasmid:707-FLPe, tet-selection
B-Bridge International, Inc.

The expression plasmid enables for FLP-mediated site specific recombination. The plasmid confers chloramphenicol resistance (708-FLPe) and propagates at...

Red/ET Hygromycin Selection Cassette, loxP-flanked, Prokaryotic and Eukaryotic (loxP-PGK-gb2-hygro-loxP)
Red/ET Hygromycin Selection Cassette, loxP-flanked, Prokaryotic and Eukaryotic (loxP-PGK-gb2-hygro-loxP)
B-Bridge International, Inc.

loxP-flanked, Prodaryotic and Eukaryotic Hygromycin Selection Cassette for use with the Red/ET Recombination System.

Red/ET Hygromycin Selection Cassette, FRT-flanked, Prokaryotic and Eukaryotic (FRT-PGK-gb2-hygro-FRT)
Red/ET Hygromycin Selection Cassette, FRT-flanked, Prokaryotic and Eukaryotic (FRT-PGK-gb2-hygro-FRT)
B-Bridge International, Inc.

FRT-flanked, Prokaryotic and Eukaryotic Hygromycin Selection Cassette for use with the Red/ET Recombination System.

Red/ET Ampicillin Selection Cassette, loxP flanked (loxP-amp-loxP)
Red/ET Ampicillin Selection Cassette, loxP flanked (loxP-amp-loxP)
B-Bridge International, Inc.

The loxP-amp-loxP cassette is designed to allow ampicillin selection in prokaryotic cells. The prokaryotic promoter gb2 driving the gene for ampicillin...

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