Mouse Wnt-9b, previously called Wnt-14b or Wnt-15, is one of at least 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved, cysteine-rich secreted glycoproteins that are important for normal developmental processes (1-3). Wnts
mainly transduce signals by binding to receptors of the Frizzled family, in conjunction with a coreceptor of the low-density lipoprotein receptor-related protein family (LRP-5 or -6) (1, 2). The 359 aa mouse Wnt-9b precursor contains a 336aa mature region with 24 conserved cysteines (3). Mature mouse Wnt-9b shows 93%, 98%, 92%, 92% and 91% aa identity with human, rat, canine, equine and bovine Wnt-9b, respectively. It is evolutionarily related to Wnt-9a and Wnt-3, which share 63% and 32% aa identity with Wnt-9B, respectively (4). Wnt-9b mRNA is expressed in late embryos and in adult kidney, with lesser amounts in brain and liver (3, 4). It appears to
direct a mesenchymal-to-epithelial transition in the kidney and other tissues (5). During kidney development, it is expressed in the ureteric bud and induces mesonephric and metanephric tubulogenesis, nephron development, and caudal extension of the Mullerian duct, acting upstream of Wnt-4 (5-7). Induction is dependent on beta-catenin activity, implicating the canonical signaling pathway (7). Mice devoid of Wnt-9b die shortly after birth due to kidney agenesis, while low expression results in small kidneys with fewer nephrons (1, 5, 6). Mutations
of Wnt-9b also cause incompletely penetrant cleft lip and palate in mice, indicating its involvement with facial midline morphogenesis (8, 9). It has weak transforming activity compared to other transforming Wnts (4).
A DNA sequence encoding mouse Wnt-9b (Met 1-Arg 359; O35468) was expressed in Chinese Hamster Ovary cells.
Based on N-terminal amino acid sequencing, the recombinant mouse Wnt-9b starts at Ala 24 and has a calculated molecular mass of approximately 36.8kD. The recombinant mouse Wnt-9b
migrates as an approximately 39-41kD protein in SDS-PAGE under reducing conditions.
Measured by its ability to induce alkaline phosphatase synthesis by C3H10T1/2 cells (ATCC# CCL-226). The typical ED 50 range is 100-400ng/ml.
Endotoxin: (same/less than)1.0 EU/1ug (LAL)
Storage and Stability:
12 months from date of receipt, 20 to 70 degrees C as supplied.
1 month, 2 to 8 degrees C under sterile conditions after reconstitution.
3 months, 20 to 70 degrees C under sterile conditions after reconstitution.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.