TROP-2, also named tumor-associated calcium signal transducer 2 (TACSTD2), GA733 tumor associated antigen, and epithelial glycoprotein-1 (EGP-1), is a type I transmembrane protein highly expressed in carcinomas.
Cell surface expression of TROP-2 is determined by flow cytometry using 488 nm wavelength excitation and monitoring emitted fluorescence with a detector optimized to collect peak emissions at 515-545 nm.
Applications:
Suitable for Flow Cytometry. Designed to quantitatively determine the percentage of cells
bearing TROP-2 within a population and qualitatively determine the density of TROP-2 on cell surfaces by flow cytometry. Other applications not tested.
Recommended Dilution:
Flow Cytometry: 10ul is added to1-2.5x10e5 cells.
Optimal dilutions to be determined by the researcher.
Hybridoma: Mouse myeloma with B cells obtained from a mouse immunized with purified, NS0-derived, recombinant human TROP-2 extracellular domain (rhTROP-2; aa 27-274; Accession # P09758).
Storage:
Lyophilized samples are stable for twelve months from date of receipt when stored at -20 degrees C to -70 degrees C. Upon reconstitution, the antibody can be stored at 2 degrees -8 degrees C for 1 month without detectable loss of activity. Reconstituted antibody can also be aliquotted and stored frozen at -20 degrees C to -70 degrees C in a manual defrost freezer for six months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
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