TREM-1 (Triggering Receptor Expressed on Myeloid Cells 1, CD354, Trem1)

TREM-1 is a 30kD monomeric transmembrane activating receptor. TREM-1 is a member of the immunoglobulin superfamily. TREM-1 is expressed at low levels in the early development of the hematopoietic system in the promonoistic stage, and at high levels on the surface of immune cells, including neutrophils, monocytes and macrofages. TREM-1 is synthesized as a 234aa precursor with a signal peptide (16aa), an extracellular domain (184aa), a transmembrane domain (29aa), and a short cytoplasmic domain (5aa). The short intracellular domain associates with a signal- transduction molecule, DNAX-activation protein 12 (DAP12), triggering the secretion of inflammatory cytokines that amplify the host response to microbial agents. TREM-1 acts in synergy with Toll-like receptor signaling pathways in amplifying the inflammatory response. Platelets express a natural ligand for TREM-1. The expression of TREM-1 is greatly upregulated on phagocytic cells in the presence of bacteria and fungi. TREM-1 has a role in sepsis, inflammatory bowel disease (IBD) and multiple sclerosis. In contrast, TREM-1 is not upregulated in samples from patients with non-infectious inflammatory conditions. During infections, receptor expression is modulated and soluble TREM-1 (sTREM-1, 17kD) is released. TREM-1 is shed from the membrane of activated phagocytes and can be found as sTREM-1 in body fluids like plasma and bronchoalveolar lavage fluid (BAL). Elavated levels of sTREM-1 have a accuracy and sensitivity in detecting microbial infections as underlying disease. Furthermore, sTREM-1 has been associated with non-infectious inflammatory conditions like major abdominal surgery, peptic ulcer disease and COPD. Applications: Suitable for use in Flow Cytometry, Western Blot, Functional Assays and Immunofluorescence. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.