Transforming Growth Factor Beta (TGFb) is a stable, multifunctional polypeptide growth factor. While specific receptors for this protein have been found on almost all mammalian cell types thus far examined, the effect of the molecule varies depending on the cell type and growth conditions. Generally, TGFb is stimulatory for cells of mesenchymal origin and inhibitory for cells of epithelial or neuroectodermal origin.
TGFb1, 2, and 3 are a closely related group of proteins (70-80% sequence homology) that are produced by many cell types and function as growth and differentiation factors. The active forms of TGFb1, 2, and 3 are disulfide-linked homodimers.
Suitable for use in Flow Cytometry. Other applications not tested.
Flow Cytometry: Neat. Use 10ul to label 5x10e5 cells.
For intracellular staining, cells must be fixed with 4% paraformaldehyde in PBS and permeabilized with 0.1% saponin in a balanced salt solution to facilitate antibody entrance into cells. Saponin must be used in all buffers used (both staining and washing steps).
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C as an undiluted liquid. Stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Freezing Allophycocyanin conjugates will result in a substantial loss of enzymatic activity.