Tumor Necrosis Factor-alpha (TNF-a) is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells. Rat TNF-a is a 17kD protein containing 157aa residues.
Suitable for use in Flow Cytometry, Western Blot, Immunohistochemistry and Neutralization. Other applications not tested.
Flow Cytometry: 10-20ug/ml
Western Blot: 1-2ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.