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TACE Activity, Fluorimetric 520, BioAssay(TM) Kit (alpha-Secretase, ADAM17, A Disintegrin and Metalloprotease)

Cat no: T0500-12

Supplier: United States Biological
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The TACE Activity Assay Kit is a homogeneous assay that can be used to detect the activity of enzyme and for screening of TACE inhibitors. It contains a 5-FAM FRET substrate, derived from a sequence surrounding the cleavage site of TACE. In the intact FRET peptide, the fluorescence of 5-FAM is quenched by a TACE Inhibitor. Active TACE cleaves FRET substrate into two separate fragments resulting in an increase of 5-FAM fluorescence which can be monitored at excitation/emission = 490nm/520nm. The long wavelength fluorescence of 5-FAM is less interfered by the autofluorescence of cell components and test compounds. Kit Components: T0500-12A: TACE substrate (4mM), 1x50ul T0500-12B: Fluoresence Standard (5-FAM), 1mM, 1x10ul T0500-12C: Assay Buffer, 1x20ml T0500-12D: TACE Inhibitor (1mM), 1x10ul T0500-12E: Stop Solution, 1x10ml Storage and Stability: Store all kit components at -20 degrees C. Protect T0500-12A and T0500-12B from light and from moisture. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: T0500-12
Size: 1Kit
References: 1. Peschon, J.J. et al. Science 282; 1281 (1998) 2. Moss, M.L. et al. Nature 385, 733 (1997). 3. Feldman, M. et al. Transplant Proc. 30, 4126 (1998). 4. Siegel, S.A. et al. Cytokine 7, 15 (1995). 5. Levin, J.I. et al. Bioor. Med. Chem. Lett 13; 2799 (2003). 6. Buxbaum, J.D. et al. J. Biol. Chem. 273, 27765 (1998). 7. Smalley, D.M. and Ley, K. J. Cell. Mol. Med. 9, 255 (2005). 8. Jin, G. et al. Anal. Biochem. 302, 269 (2002).

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