The synapsins are a family of proteins that have long been implicated in the regulation of neurotransmitter release at synapses. Specifically, they are thought to be involved in regulating the number of synaptic vesicles available for release via Exocytosis. All synapsins contain a short amino-terminal domain that is highly conserved and phosphorylated by PKA or CaM kinase I. Phosphorylation of synapsin amino-terminal domain at serine 9 inhibits its binding tophospholipids and dissociates synapsins from synaptic vesicles.
Suitable for use in Western Blot. Other applications not tested.
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.
Storage and Stability:
For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.