Synapsins are involved in neurotransmitter release. Synapsin I cross-links synaptic vesicles and cytoskeleton. The interactions of the protein with actin filaments and synaptic vesicle are regulated by phosphorylation. Synapsin I has many potential phosphorylation sites available to different enzymes, serines 62 and 67 are phosphorylated by extracellular signal-regulated kinases 1 and 2 of the mitogen-activated protein (MAP) kinase superfamily. Map-kinase phosphorylation of synapsins is a key step in the modulation of neurotransmitter release by brain-derived neurotrophic factor.
Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Western Blot: 1:500-1:1000
Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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