Spleen tyrosine kinase (SYK) is a 70-80kD, ubiquitously expressed member of the protein kinase superfamily, the tyrosine protein kinase family, and the SYK/ZAP70 subfamily of proteins. Human SYK is 635aa in length and contains two SH2 domains (aa14106 and aa168259 and one protein kinase domain (aa371-631). A splicing variant produces a second isoform that has a deletion of aa371-631 found in the longer isoform. Human SYK shares 92% and 91% aa sequence identity with mouse and rat SYK, respectively. Functionally, SYK is a positive effector of Bcell antigen receptorstimulated
Suitable for use in Western Blot. Other applications not tested.
Western Blot: 0.1ug/ml detects human SYK phosphorylated at Y525/Y526 in Ramos human Burkitt