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Cat no: S8999-04

Supplier: United States Biological
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Applications: Suitable for use in Flow Cytometry and Immunohistochemistry (frozen). Other applications not tested. Recommended Dilution: Flow Cytometry: 1:20; 50ul labels 10e6 cells in 100ul Immunohistochemistry (frozen): 1:10-1:50 Optimal dilutions to be determined by the researcher. Hybridoma: P3X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: S8999-04
Reactivities: Porcine
Hosts: Mouse
Applications: Flow Cytometry, Immunohistochemistry
Size: 2ml
Form: Supplied as a liquid, 0.2M Tris/HCl, pH 7.4, 5-10% fetal calf serum, 0.09% sodium azide.
P type: Mab
Isotype: IgM
Purity: Supernatant
References: 1. Haverson K. et al. (1994) Characterization of monoclonal antibodies specific for monocytes, macrophages and granulocytes from porcine peripheral blood and mucosal tissues. J. Immunol. Methods. 170: 233-245. 2. Saalmuller, A. (1996) Characterization of swine leukocyte differentiation antigens. Immunol. Today 17: 352-354. 3. Summerfield, A. et al. (2001) Induction of apoptosis in bone marrow neutrophil-lineage cells by classical swine fever virus. J. Gen. Virol. 82: 1309-1318.
Additional info: Recognizes porcine SWC8.

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