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Cat no: S8999-03

Supplier: United States Biological
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Applications: Suitable for use in Flow Cytometry, Immunohistochemistry (frozen), and Immunoprecipitation. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:25-1:50; 10ul labels 10e6 cells in 100ul Optimal dilutions to be determined by the researcher. Hybridoma: X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: S8999-03
Reactivities: Porcine
Hosts: Mouse
Applications: Flow Cytometry, Immunohistochemistry, Immunoprecipitation
Size: 250ug
Form: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
P type: Mab
Isotype: IgG2a
Purity: Purified by Protein G affinity chromatography.
References: 1. Bullido, R. et al. (1999) Monoclonal antibodies 2F6/8 and 2A10/8 recognize a porcine antigen (SWC7) expressed on B cells and activated T cells. J. Immunol. Methods. 222:1-11. 2. Boersma, W.J.A.et al. (2001) Summary of workshop findings for porcine B-cell markers. Vet. Immunol. Immunopathol. 80:63-78. 3. Saalmuller, A.et al. (1998) Overview of the second international workshop to define swine cluster of differentiation (CD) antigens. Vet. Immunol. Immunopathol. 60: 207-228. 4. Denham, S.et al. (1998) Monoclonal antibodies putatively identifying porcine B cells. Vet. Immunol. Immunopathol. 60: 317-328.
Additional info: Recognizes the porcine SWC7 cell surface antigen which is expressed by activated B lymphocytes and a subset of CD3+ T lymphocytes.This clone immunoprecipitates a dimeric molecule with an apparent molecular size of 40kD (non-reduced) or 24kD (reduced).

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