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SWC6 Null Cells

Cat no: S8999-02

Supplier: United States Biological
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Applications: Suitable for use in ELISA, Flow Cytometry, Immunohistochemistry (frozen and paraffin), and Immunoprecipitation. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:10-1:50; 10ul labels 10e6 cells in 100ul Optimal dilutions to be determined by the researcher. Hybridoma: Y3.Ag1.2.3 rat myeloma cells with spleen cells from PVG rats. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: S8999-02
Reactivities: Porcine
Hosts: Rat
Applications: ELISA, Flow Cytometry, Immunohistochemistry, Immunoprecipitation
Size: 2ml
Form: Supplied as a liquid, 0.2M Tris/HCl, pH 7.4, 5-10% fetal calf serum, 0.09% sodium azide.
P type: Mab
Isotype: IgG2a
Purity: Supernatant
References: 1. Binns, R.M. et al. (1992) Subsets of null and gamma delta T-cell receptor+ T lymphocytes in the blood of young pigs identified by specific monoclonal antibodies Immunology 77: 219-227. 2. Binns, R.M. et al. (1994) The Null/gamma delta TCR+ T cell family in the pig Vet. Immunol. Immunopathol. 43: 69-77.
Additional info: Recognizes the porcine SWC6 cell surface antigen, expressed by all 'null' lymphocytes.

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