Apoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members in the TNF family and prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family that binds to and inhibits cell death proteases. A novel IAP protein was recently identified and designated survivin, apoptosis inhibitor 4 (API4), and TIAP. Survivin:TIAP interacted with the processed form of caspase-3 and inhibited its proteolytic activity. Survivin:TIAP is predominantly expressed in tissues of embryos, transformed cell lines, and many human cancers and lymphomas.
Suitable for use in Western Blot. Other applications not tested.
Western Blot: 1-2ug/ml
Optimal dilutions to be determined by the researcher.
Mouse spleen tissue lysate.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.