Home  >  Products  >  SUPT16H (FACT Complex Subunit SPT16, Facilitates Chromatin Transcription Complex Subunit SPT16, hSPT16, FACT 140kD Subunit, FACTp140, Chromatin-specific Transcription Elongation Factor 140kD Subunit, FACT140, FACTP140)

SUPT16H (FACT Complex Subunit SPT16, Facilitates Chromatin Transcription Complex Subunit SPT16, hSPT16, FACT 140kD Subunit, FACTp140, Chromatin-specific Transcription Elongation Factor 140kD Subunit, FACT140, FACTP140)

Cat no: S8091-21


Supplier: United States Biological
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Transcription of protein-coding genes can be reconstituted on naked DNA with only the general transcription factors and RNA polymerase II. However, this minimal system cannot transcribe DNA packaged into chromatin, indicating that accessory factors may facilitate access to DNA. One such factor, FACT (facilitates chromatin transcription), interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT is composed of an 80kD subunit and a 140kD subunit, the latter of which is the protein encoded by this gene. Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (Formalin fixed paraffin embedded): 20ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: S8091-21
Reactivities: Human
Hosts: Rabbit
Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
Size: 50ug
Form: Supplied as a liquid in PBS, 0.08% sodium azide.
P type: Pab
Purity: Purified
Additional info: Recognizes human SUPT16H. Species sequence homology: Mouse

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