Superoxide dismutase comprises a family of metalloenzymes that catalyze the oxido-reduction of superoxide anion to H2O2. Manganese superoxide dismutase (SOD2) is encoded by nuclear chromatin, synthesized in the cytosol, and imported posttranslationally into the mitochondrial matrix (1). It has been shown that SOD2 activity in tumor cells is lower than that in their normal counterparts. results suggest that the reduced level of SOD2 activity observed in human tumor cells is not due to a defect in the primary structure of the SOD2 protein, a change in the dosage of the SOD2 gene, or a decrease in the stability of SOD2 mRNA in tumor cells but rather is due to a defect or defects in the expression of the gene (2). SOD2 is one of the major cellular defense enzymes that protects against toxic effects of superoxide radicals. Overexpression of human SOD2 has been shown to inhibit radiation-induced neoplastic transformation, suppress malignancy of cancer cells, and increase tolerance to various toxic agents (3).
Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Western Blot: 1:1000-1:2000
Immunohistochemistry: 1:100-1:250
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Manufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
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