Suitable for use in ELISA and Western Blot. Other applications not tested.
Western Blot: 0.01-0.03ug/ml. Approximately18kD band observed in mouse brain and rat spinal cord lysates and in purified membranes of cell line HeLa (calculated MW of 15.9kD according to human NP_000445.1, mouse NP_035564.1 and rat NP_058746.1)
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.