SAE1 is a 35.5kD nuclear protein belonging to Ubiquitin E1 family. SAE1 acts as an E1 activating enzyme for SUMO1, SUMO2 and SUMO3. It mediates ATP-dependent activation of SUMO proteins and formation of a thio-ester linkage with a conserved cysteine residue on SAE2. SAE1 is broadly expressed in a wide range of tissues.
Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.
Immunohistochemistry (paraffin): 5ug/ml
Western Blot: 1:500-1:2,000
Optimal dilutions to be determined by the researcher.
Positive Control Tissue:
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.