SUMF1 activates sulfatases by converting their active site residue cysteine to formylglycine.
Suitable for use in ELISA and Western Blot. Other applications not tested.
Western Blot: 0.5-2ug/ml detects ~37-40kDa band observed in Mouse Pancreas, Mouse Eye and Human Kidney lysates (calculated MW of 40.6kDa according to Human NP_877437.2 and 40.6kDa according to Mouse NP_666049.2).
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.