Home  >  Products  >  SUMF1 (Sulfatase Modifying Factor 1, AAPA3037, FGE, MGC131853, MGC150436, C-alpha-Formylglycine-Generating Enzyme)
SUMF1 (Sulfatase Modifying Factor 1, AAPA3037, FGE, MGC131853, MGC150436, C-alpha-Formylglycine-Generating Enzyme)

SUMF1 (Sulfatase Modifying Factor 1, AAPA3037, FGE, MGC131853, MGC150436, C-alpha-Formylglycine-Generating Enzyme)

Cat no: S8016-09

Supplier: United States Biological
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SUMF1 activates sulfatases by converting their active site residue cysteine to formylglycine. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:16,000 Western Blot: 0.5-2ug/ml detects ~37-40kDa band observed in Mouse Pancreas, Mouse Eye and Human Kidney lysates (calculated MW of 40.6kDa according to Human NP_877437.2 and 40.6kDa according to Mouse NP_666049.2). Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: S8016-09
Reactivities: Human, Mouse
Hosts: Goat
Applications: ELISA, Western Blot
Size: 100ug
Form: Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.
P type: Pab
Isotype: IgG
Purity: Purified by affinity chromatography.
References: 1. Oshikawa M, Usami R, Kato S. Characterization of the arylsulfatase I (ARSI) gene preferentially expressed in the human retinal pigment epithelium cell line ARPE-19. Molecular vision 2009 15 : 482-94. PMID: 19262745
Additional info: Recognizes human SUMF1. Species crossreactivity: mouse. Sequence Homology: rat, bovine, canine.

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