The sulphonamides are competitive inhibitors of p-aminobenzoic acid in the folic acid metabolism cycle and have a bacteriostatic effect on a broad range of Gram-positive and Gram-negative organisms. The sulphonamides are distributed widely throughout all organs and tissues; and are metabolized primarily by the liver, producing inactive acetylated and glucuronide forms, which are excreted in urine (28). Sulphonamides are used extensively in veterinary medicine and their use in food producing animals could result in potentially harmful concentrations in tissue, organs and milk. The potential risk is reduced by withdrawal of the drug for a fixed period before slaughter, although residual levels may still remain.
Applications:
Suitable for use in ELISA and in the development of immunoassays or immunoaffinity purification columns. Other applications not tested.
Recommended Dilution:
ELISA: 1.25ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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