STYX belongs to the protein-tyrosine phosphatase family, Non-receptor class subfamily. It contains one tyrosine-protein phosphatase domain and is a probable pseudophosphatase. It has a Gly residue instead of a conserved Cys residue in the dsPTPase catalytic loop which renders it catalytically inactive as a phosphatase. The binding pocket is however sufficiently preserved to bind phosphorylated substrates, and may be protect them from phosphatases. STYX seems to play a role in spermiogenesis.
Suitable for use in Western Blot. Other applications not tested.
Western Blot: 1:1000-1:2000; Band is observed at ~25.5kD using STYX-transfected 293T lysate
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.