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Stromal Antigen 2 (SA2, SA-2, STAG2, Cohesin Subunit SA-2, SCC3 Homolog 2)

Stromal Antigen 2 (SA2, SA-2, STAG2, Cohesin Subunit SA-2, SCC3 Homolog 2)

Cat no: S7975-11B

Supplier: United States Biological
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The cohesin complex consists of a heterodimer between SMC1 (SMC1A or B) and SMC3, bound by additional RAD21 and STAG proteins (STAG1, 2 or 3) (1,2). These proteins form a ring-like structure that mediates the cohesion of two sister chromatids after DNA replication in S phase (1,2). RAD21 and STAG2 are phosphorylated by Polo-like kinase (PLK) during prophase, which leads to the dissociation of cohesin complexes from the chromosome arms; however, cohesin remains bound to centromeres until anaphase (3,4). RAD21 is cleaved by separin/ESPL1 in anaphase, which leads to dissociation of the remaining cohesin from centromeres, enabling sister chromatids to segregate during mitosis (5). RAD21 is also cleaved by caspase-3 and caspase-7 during apoptosis, resulting in a 64kD carboxy-terminal cleavage product that translocates to the cytoplasm and may help to trigger apoptosis (6,7). In addition to mediating cohesion of sister chromatids, the cohesin complex plays important roles in gene regulation and DNA repair, as SMC1 and SMC3 are both phosphorylated by ATM and ATR kinases upon DNA damage (1,2). Applications: Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: S7975-11B
Reactivities: Human, Xenopus/Amphibian
Hosts: Goat
Applications: Immunocytochemistry, Western Blot
Size: 100ul
Form: As reported
P type: Pab
Purity: Purified by immunoaffinity chromatography.
References: General References: 1. Peters, J.M. et al. (2008) Genes Dev 22, 3089-114, 2. Barbero, J.L. (2009) Cell Mol Life Sci 66, 2025-35, 3. Hoque, M.T. and Ishikawa, F. (2001) J Biol Chem 276, 5059-67, 4. Hauf, S. et al. (2005) PLoS Biol 3, e69, 5. Hauf, S. et al. (2001) Science 293, 1320-3, 6. Pati, D. et al. (2002) Mol Cell Biol 22, 8267-77, 7. Chen, F. et al. (2002) J Biol Chem 277, 16775-81.
Additional info: Specific for human SA2 protein. Species Crossreactivity: Xenopus. Other species have not been tested.

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