LKB1 (also STK11 and JBS) is a 55kD member of the CAMK Ser/Thr protein kinase family. It is ubiquitously expressed, and considered to be a tumor suppressor. LKB1 is both nuclear and cytoplasmic, and appears to contribute to cell polarization, G1 cell cycle arrest and Wnt signaling. It is known to complex with STRAD and CAB39/MO25, and phosphorylate PTEN plus p53. Human LKB1 is 433aa in length. It contains a protein kinase domain (aa49-309) and a prenylation motif (CysLysGlnGln) over aa430-433. There are two potential isoforms of LKB1. One shows a nine aa insertion after Tyr126, while another shows a 34aa substitution for aa371-433. Over aa242-433, human and mouse LKB1 are identical in aa sequence.
Suitable for use in Western Blot. Other applications not tested.
Western Blot: 0.3-1ug/ml
Optimal dilutions to be determined by the researcher.
Human Ovary lysate
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.