Human STAMP2, also called Steap4 or TIARP (TNF-alpha-induced adipose-related protein), is a 55kD, 459aa, 6-transmembrane (TM) member of the Steap family of metalloreductases. It is induced by androgens in androgen-regulated prostate cells, and is upregulated during adipocyte differentiation by TNF-alpha. Full-length human and mouse STAMP2 share 80% aa identity. A 283aa variant lacks a portion of the oxidoreductase domain and three TM domains. STAMP2 is present in the Golgi complex and early endosomes in addition to the cell surface.
Suitable for use in Flow Cytometry. Other applications not tested.
Flow Cytometry: 25ug/ml; Use 10ul to label 1-2.5x10e5 cells in total reaction volume (same/less than)200ul.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.