Studies indicate that op18 may function as an intracellular relay by integrating several signalling pathways such as those involved in cell proliferation and differentiation. In addition, op18 is involved in the regulation of the microtubule filament system by destabilizing microtubules where it prevents assembly, and promotes disassembly of microtubules.
Research also indicates that op18 is a potential target of the ASK1-p38 MAP kinase cascade suggesting that the ASK1-p38 cascade may regulate microtubule dynmamics through op18.
Op18, while ubiquitously expressed in normal tissues, is highly expressed in a variety of cancers including leukemia, prostate cancer, ovarian carcinoma and breast carcinoma. It has therefore been hypothesized that op18 may be a potential target for anti-cancer therapeutics.
Applications:
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution:
Western Blot: 0.5-1ug/ml, detects a band of approximately 15kD in EL4 cell lysates.
Optimal dilution to be determined by researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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