STAT1alpha (Signal transducer and activator of transcription 1 alpha) is a 91kD protein belonging to the transcription factor STAT family. Members of the STAT family respond to cytokines and growth factors and are phosphorylated by receptor associated kinase to form homo- or hetro-dimers. These then translocate to the cell nucleus to act as transcription activators. STAT1 is activated by a number of different ligands including the IFN, gp130 and receptor tyrosine kinase families. STAT1 has two different forms, a 91kD form (STAT1alpha) and an 84kD form (STAT1beta), This antibody is specific for the 91kD form, STAT1alpha and does not react with STAT1beta.
Suitable for use in Immunohistochemistry (frozen), Immunoprecipitation, and Western Blot. Other applications not tested.
Immunohistochemistry (frozen): 10ug/ml
Western Blot: 1:1,000-1:2,000
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.