STATs (signal transducers and activators of transcription) are a family of cytoplasmic latent transcription factors that are activated to regulate gene expression in response to a large number of extracellular signaling polypeptides including cytokines, interferons, and growth factors. After phosphorylation by JAK tyrosine kinases, STATs enter the nucleus to regulate transcription of many different genes. Stat6 is rapidly tyrosine phosphorylated following stimulation of appropriate cell lines with IL-3, IL-4, epidermal growth factor (EGF), but is not detectably phosphorylated following stimulation with IL-2, IL-12, or erythropoietin.
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Western Blot: 1-2ug/ml
Optimal dilution to be determined by researcher.
Recommended Positive Control:
NIH 3T3 cells treated with either anisomycin (10ug/ml) or EGF (100ng/ml)
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.