A variety of cytokines and growth factors act through an induction of gene expression mediated by family of latent transcription factors called STAT (Signal Transducers and Activators of Transcription) proteins. Cytokines, hormones and growth factors proceed through a sequence of steps that includes binding to receptors in the cytokine receptor superfamily followed by the activation of the JAK family of tyrosine kinases, which results in the activation of STAT proteins. STAT6, a 110kD protein, translocates into the nucleus following activation via phosphorylation at its Tyr641. STAT6 is required for responsiveness to IL-4 and IL-13 induced gene expression, where it is phosphorylated by JAK kinase at Tyr-641 on the C-terminal side of the SH2 domain and dimerized to manipulate transcription machinery by binding to p300/CBP. STAT6 interacts with STAT2 and p48 to form transcriptionally active complexes in B-cells after being activated by IFN-a. Protein phosphatase 2A is also involved in regulation of IL-4- mediated STAT6 signaling.
Suitable for use in Western Blot. Other applications not tested.
Western Blot: 0.5-2ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.