Signal Transducer and Activator of Transcription 6 (STAT6) is 119kD mediator of transcription in response to IL-4 and IL-13 stimulation. It resides in the cytoplasm in a latent form as a monomer protein. Active IL-4 engages SH2 binding domain of STAT6 that results in STAT6 phosphorylation at Tyr641 residue. Phosphorylated monomeric STAT6 dimerize via their N- terminal domain. Consequently dimeric STAT6 is translocated into the nucleus, binds target DNA sequence, and promotes transcription. It was demonstrated that normal STAT6 function is critical for commitment of CD4+ T cells to the Th2 phenotype and IgE response. Thus, mice deficient in STAT6 failed to produce IgE, were deficient in Th2 differentiation, and did not show allergic airway response.
Suitable for use in Western Blot. Other applications not tested.
Western Blot: 0.5-2ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.