STATs (signal transducing activators of transcription) are mediators involved in cytokine signaling. Being the principle substrates of JAK kinases, phosphorylated STATs form head-to-tail homodimers. In response to a specific cytokine signal, STATs move to the nucleus and induce transcription. STAT6 is activated primarily by IL-4 and IL-13.
Suitable for use in ELISA and Western Blot. Other applications not tested.
Western Blot: 1ug/ml for HRP/ECL detection
Optimal dilutions to be determined by the researcher.
Positive Control (supplied):
Cell lysate of 2 x 10e6 untreated HepG2 cells. Supplied as a lyophilized powder. Reconstitute with 200ul sterile ddH2O.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Reconstitute with sterile ddH2O, sterile 40-50% glycerol, aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.