A variety of cytokines and growth factors act through an induction of gene expression mediated by a family of latent transcription factors called STAT (Signal Transducers and Activators of Transcription) proteins. Cytokines, hormones and growth factors proceed through a sequence of steps that includes binding to receptors in the cytokine receptor superfamily followed by the activation of the JAK family of tyrosine kinases, which results in the activation of STAT proteins. The activated STATs then become dimerized and translocated into the nucleus where they initiate specific gene transcription. STAT 5 regulates growth, differentiation, and survival of mammary, hematopoietic cells, hematopoietic malignancies, or carcinogenesis. Constitutive activation of STAT 5 may contribute to tumorigenesis, thus dephosphorylation of STAT 5 may serve as a therapeutic strategy.
Suitable for use in Western Blot. Other applications not tested.
Western Blot: 0.5-2ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.