STATs (Signal Transducers and Activators of transcription) are a family of cytoplasmic latent transcription factors that are activated to regulate gene expression in response to a large number of extracellular signaling polypeptides including cytokines, interferons, and growth factors. After phosphorylation by JAK tyrosine kinases, STATs proceed to dimerize and enter the nucleus to regulate transcription of many different genes. Among the seven STATs (Stat-1, Stat-2, Stat-3, Stat-4, Stat-5a, Stat-5b, and Stat-6), Stat-1, Stat-3, Stat-5a, and Stat-5b have a wide activation profile (1,2). STAT1 is activated by many different ligands including IFN family (IFN-a, IFN-b, IFN-g and IL-10), gp130 family (IL-6, IL-11, LIF, CNTF, and CSF), and receptor tyrosine kinases (EGF, PDGF, and CSF-1). STAT1 has two forms, the 91kD STAT1a and the 84kD STAT1b, are encoded by the same gene with splicing variant.
Applications:
Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilution:
ELISA: 0.1-1ug/ml
Western Blot: 0.5-2ug/ml
Immunoprecipitation: 3-5ug/extract from 10e6 cells
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.