Suitable for use in ELISA, Western Blot. Other applications not tested.
Peptide ELISA: 1:64,000.
Western Blot: 0.3-1ug/ml. Approx 48kD band observed in Human Brain (Cerebellum) lysates (calculated MW of 53.0kD according to NP_005477.1). Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Corresponding peptide is available for Peptide Blocking studies. See S6504-39-P. Antibody