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Sprouty Homolog 1 (Protein Sprouty Homolog 1, Spry-1, SPRY1)

Cat no: S5620-01E1

Supplier: United States Biological
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SPRY1 is a 34-38kD member of the sprouty family of proteins. It is widely expressed, being found in multiple embryonic and adult tissues. SPRY1 is considered a negative regulator of cellular signaling. In particular, it appears to both inhibit MAP kinase signaling following RTK activation, and block TCR signaling following antigen activation. It interacts with a number of molecules, including PLC-g 1, LAT, CBL, caveolin-1 and SPRY2. Human SPRY1 is 319aa in length. It contains one CBL-TKB binding site aa51-57 that is phosphorylated at Tyr53, a Ser-rich region aa112-131, and a Cys-rich domain aa181-306 that mediates intracellular translocation. SPRY1 undergoes serine phosphorylation, ubiquitination and palmitoylation, the latter which induces SPRY1 to associate with cell membranes. Over aa1-178, human SPRY1 shares 76% aa identity with mouse SPRY1. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.5ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: S5620-01E1
Reactivities: Human
Hosts: Mouse
Size: 100ug
Form: Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml.
P type: Mab
Isotype: IgG2B
Purity: Purified by Protein A/G affinity chromatography.
Additional info: Recognizes human SPRY1. Species sequence homology: mouse (76%). No cross-reactivity with recombinant human SPRY2, 3, or 4.

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