Testican 2 (also SPOCK2) is a 60-140kD, secreted multidomain proteoglycan that belongs to the BM-40/SPARC family of extracellular proteins. It is expressed by neurons (both excitatory and GABAergic) plus brain endothelial cells, and appears to abrogate testican 1 and 3 mediated inhibition of MT1- and MT3-MMP activity. Mature human Testican 2 is 402aa in length (aa23- 424). It contains a unique 66aa N-terminus, followed by a Kazal-like follistatin domain (aa89- 192), a collagen-binding domain (aa210-219), a Ca++ binding region, one thyroglobulin-like segment (aa310-376) and an acidic C-terminus that contains two potential GAG attachment sites at Ser383 and Ser388. The wide variation in native MW reflects the capabilities of differing cells to add glycan adducts to the molecule. Typically, it appears to lie belween 80-125kD. There are potential splice variants. One shows an alternative start site at Met110, a second shows a deletion of (aa64-66), while a third contains a 14aa substitution for (aa64-242). Mature human Testican 2 is 94% aa identical to mouse and rat Testican 2.
Applications:
Suitable for use in ELISA, Western Blot, Immunoprecipitation. Other applications not tested.
Recommended Dilution:
Direct ELISA: 0.5-1ug/ml
Western Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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