Spleen focus forming virus [SFFV] proviral integration oncogene (spi-1) is a mammalian transcription factor critically involved in a widespread role throughout hematopoiesis in many species. The zebrafish orthologue of spi-1 marks a population of rostral lateral plate mesoderm cells during development fated for myeloid differentiation. Spi-1 gene expression has been demonstrated to be largely recapitulated in transgenic zebrafish embyos via injection of an upstream promoter. Zebrafish are a powerful tool for the study of vertebrate hematopoiesis, as a result of possessing a robust primitive myeloid pathway that generates both granulocytes and macrophages. Previously known as pu1, spi-1 has been demonstrated strong functional conservation between zebrafish primitive myelopoiesis and mammalian definitive myelopoiesis.
Suitable for use in ELISA. Other applications not tested.
Optimal dilutions to be determined by the researcher.
Control Peptide: S5490-10Q
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.