Spleen focus forming virus [SFFV] proviral integration oncogene (spi-1) is a mammalian transcription factor critically involved in a widespread role throughout hematopoiesis in many species. The zebrafish orthologue of spi-1 marks a population of rostral lateral plate mesoderm cells during development fated for myeloid differentiation. Spi-1 gene expression has been demonstrated to be largely recapitulated in transgenic zebrafish embyos via injection of an upstream promoter. Zebrafish are a powerful tool for the study of vertebrate hematopoiesis, as a result of possessing a robust primitive myeloid pathway that generates both granulocytes and macrophages. Previously known as pu1, spi-1 has been demonstrated strong functional conservation between zebrafish primitive myelopoiesis and mammalian definitive myelopoiesis.
Suitable for use in ELISA and Western Blot. Other applications not tested.
Western Blot: 1:500-1:1000
Optimal dilutions to be determined by the researcher.
Control Peptide: S5490-10P
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.