SphK1 catalyzes the phosphorylation of sphingosine 1-phosphate; an important lipid mediator with multiple functions. In light of this, SphK1 has been implicated in regulation of Ca2+ homoeostasis, inflammation, immunity and cancer growth. However, its central function is thought to be in the development of the cardiovascular and central nervous system. SphK1 has a molecular weight of 42kD.
Suitable for use in Immunohistochemistry (paraffin). Other applications not tested.
Immunohistochemistry (paraffin): 5-15ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.