EDG3 has been reported in all tissues, with highest levels in heart, placenta, kidney, and liver. ESTs have been isolated from placenta and germ cell tumor libraries.
Suitable for use in ELISA and Immunohistochemistry. Other applications not tested.
Immunohistochemistry: 10-20ug/ml. Formalin-fixed paraffin-embedded (FFPE) tissues, after heat induced antigen retrieval in pH 6.0 citrate buffer.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.