Alpha Fodrin is a member of the spectrin family of widely distributed filamentous proteins that form a supporting and organizing scaffold for cell membranes (1). Non-erythroid alpha spectrin, also called Alpha Fodrin, is a major cortical cytoskeletal protein that is implicated in the establishment of specialized membrane-cytoskeletal domains in differentiating cells (2, 3). Cleavage of Alpha Fodrin accompanies apoptosis, induced by activation via the CD3/T cell receptor complex, ligation of the Fas (CD95) molecule and other Fas-expressing cells; it may also be induced by the treatment of cells with staurosporine, dexamethasone, or synthetic ceramide (4). The major products of cleavage are amino-terminal and carboxy-terminal fragments. Antibodies against Alpha Fodrin are observed in diseases characterized by chronic apoptosis (5, 6).
Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry and Immunoprecipitation. Other applications not tested.
Western Blot: 1:10,000-1:50,000
Immunohistochemistry (Paraffin): 1:500-1:1000
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Manufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.