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Spectrin alpha chain, erythrocyte (Spectrin, alpha, erythrocytic 1, EL2, Erythroid alpha-spectrin, HPP, HS3, Spectrin alpha chain, erythrocyte, SPH3, SPTA)

Spectrin alpha chain, erythrocyte (Spectrin, alpha, erythrocytic 1, EL2, Erythroid alpha-spectrin, HPP, HS3, Spectrin alpha chain, erythrocyte, SPH3, SPTA)

Cat no: S5381-03C

Supplier: United States Biological
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Spectrin is the predominant component of the membrane skeleton of the red blood cell and is essential in determining shape and deformability of the membrane. It consists of 2 nonidentical subunits, alpha (MW 240,000) and beta (MW 225,000). Spectrin is present in the red cell membrane in a tetrameric or possibly higher polymeric form through head to head self association of heterodimers that are linked by actin polymers and protein 4.1 to form a 2 dimensional network. Applications: Suitable for use in Immunofluorescence, ELISA, Western Blot, and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunofluorescence: 1:100 -1:500 ELISA: 1:105-1:106 Western Blot: 1:102-1:103 Immunohistochemistry: 1:10-1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: S5381-03C
Reactivities: Human, Mouse, Rat
Hosts: Mouse
Applications: ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Size: 200ul
Form: Supplied as a liquid in PBS, 0.1% sodium azide.
P type: Mab
Isotype: IgG1
Purity: Purified by ammonium sulfate precipitation and dialysis.
Additional info: Recognizes alpha I spectrin SH3 domain. Does not cross react with alpha II (non-erythroid) spectrin SH3 domain or recombinant human fodrin SH3 domain. Species Crossreactivity: human, rat, mouse

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