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Specific Intracellular Adhesion Molecule Grabbing Nonintegrin R1 (SIGN-R1)

Cat no: S1013-69

Supplier: United States Biological
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Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry: Frozen 1:25-1:50. Optimal dilutions to be determined by the researcher. Hybridoma: Hybridization of P3-X63-Ag8.563 myeloma cells with spleen cells from immunized rats. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: S1013-69
Reactivities: Mouse
Hosts: Rat
Applications: Flow Cytometry, Immunohistochemistry
Size: 250ug
Form: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide
P type: Mab
Isotype: IgM
Purity: Purified by ammonium sulfate precipitation.
References: 1. van Vliet, E., et al., (1985),
Additional info: Recognizes mouse SIGN-R1, a C-type lectin that has been identified as a murine homolog of the human CD-SIGN related molecule, L-SIGN. Mouse SIGN-R1 is specifically expressed on marginal zone macrophages in the spleen, medullary and subcapsular sinus macrophages in the lymph nodes, and at lower levels on liver sinusoidal endothelial cells. SIGN-R1 has not been detected on murine dendritic cell populations. Blocks the uptake of neutral polysaccharides by macrophages.

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