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SP19 (pan Sodium Channel)

SP19 (pan Sodium Channel)

Cat no: S5368

Supplier: United States Biological
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Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:200 using ECL on rat brain membranes Immunohistochemistry: Frozen mouse brain sections; Paraffin mouse spinal cord sections. Note: All procedures that are going to receive a full-length protein should be performed at 4 degrees C, and the following protease inhibitor mixture should be used: pepstatin A (1ug/ml), leupeptin (1ug/ml), aprotinin (1ug/ml), Pefabloc SC (0.2mM), benzamidine (0.1mg/ml), and calpain inhibitors I and II (8ug/ml each). Optimal dilutions to be determined by the researcher. Included Negative Control: S5368A: SP19 (pan Sodium Channel), Rat, Control Peptide Supplied as a lyophilized powder. Reconstitute in 100ul sterile ddH2O. Preincubate 1ug of peptide with 1ug of antibody for one hour at RT. *Recommended Secondary Antibodies I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb I1904-46Q: IgG, H&L (HRP) Pab Gt xRb Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: S5368
Reactivities: Mouse, Rat
Hosts: Rabbit
Applications: Immunohistochemistry, Western Blot
Size: 50ul
Form: Supplied as a lyophilized powder from PBS, pH 7.4, 1% BSA, 0.05% sodium azide. Reconstitute with 50ul sterile ddH2O.
P type: Pab
Isotype: IgG
Purity: Purified by affinity chromatography.
References: 1. Yue, L. et al. (1999) Circ Res. 84, 776. 2. Anis, Y. et al. (1999) J. Biol. Chem. 274, 7431. 3. Hanani, M. et al. (2000) Am J. Physiol. 278, G644.
Additional info: Recognizes the alpha subunits of SP19 (pan Sodium Channel). Species Crossreactivity: Rat and mouse

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