Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Western Blot: 1:200 using ECL on rat brain membranes
Immunohistochemistry: Frozen mouse brain sections; Paraffin mouse spinal cord sections.
Note: All procedures that are going to receive a full-length protein should be performed at 4 degrees C, and the following protease inhibitor mixture should be used: pepstatin A (1ug/ml), leupeptin (1ug/ml), aprotinin (1ug/ml), Pefabloc SC (0.2mM), benzamidine (0.1mg/ml), and calpain inhibitors I and II (8ug/ml each).
Optimal dilutions to be determined by the researcher.
Included Negative Control:
S5368A: SP19 (pan Sodium Channel), Rat, Control Peptide
Supplied as a lyophilized powder. Reconstitute in 100ul sterile ddH2O. Preincubate 1ug of peptide with 1ug of antibody for one hour at RT.
*Recommended Secondary Antibodies
I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb
I1904-46Q: IgG, H&L (HRP) Pab Gt xRb
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.