SOX-9 is a member of the family of SOX (Sry-type high mobility group box) gene that were first identified on the basis of region with high homology to that of Sry (Sex-determining region Y). It is a transcription factor with a high mobility group DNA-binding domain that is expressed in all prechondrocytic and chondrocytic cells during embryonic development in a pattern that close parallels that of the gene for type II collagen. Phosphorylation of SOX-9 by PKA increases its DNA binding and transcriptional activity. SOX-9 regulates expression of chondrocyte phenotype related genes. IL-1 and TNF-a can cause a marked and rapid decrease in the levels of SOX-9 mRNA and/or protein in chondrocytes, thus strongly inhibit the expression of genes for cartilage extracellular matrix proteins. The down-regulation of SOX-9 may have a crucial role in inhibiting expression of the cartilage phenotype in inflammatory joint diseases.
Suitable for use in ELISA and Western Blot. Other applications not tested.
Western Blot: 1:500-1:1000
Optimal dilutions to be determined by the researcher.
Control Peptide: S5364-28S
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.