Home  >  Products  >  Solute Carrier Family 12a3 (Slc12a3, Sodium/chloride transporters, member 3, Na-Cl symporter, NCC, Solute carrier family 12 member 3, Thiazide- sensitive sodium-chloride cotransporter, Tsc, TSC)
Solute Carrier Family 12a3 (Slc12a3, Sodium/chloride transporters, member 3, Na-Cl symporter, NCC, Solute carrier family 12 member 3, Thiazide- sensitive sodium-chloride cotransporter, Tsc, TSC)

Solute Carrier Family 12a3 (Slc12a3, Sodium/chloride transporters, member 3, Na-Cl symporter, NCC, Solute carrier family 12 member 3, Thiazide- sensitive sodium-chloride cotransporter, Tsc, TSC)

Cat no: S5329-13B

Supplier: United States Biological
Star_fadedStar_fadedStar_fadedStar_fadedStar_faded
0 reviews | Write a Review Pencil
Read More on Supplier Website
Slc12a3, a thiazide-sensitive NaCl co-transporter, is found on the apical membrane of the distal convoluted tubule, where it is the principal mediator of Na+ and Clreabsorption in this segment of the nephron. It is activated by phosphorylation, and has been implicated in renal NaCl and K+ homeostasis (1). Regulation of Slc12a3 expression and phosphorylation by dietary NACl restriction appears to involve SGK1(1). In experiments with angiotensin II-infused mice, increased sensitivity to Ang II may involved over-activity of Slc12a3 (2). Therefore, Slc12a3 is the target of thiazide diuretics used in the treatment of hypertension (1). Molecular experiments have also shown that Slc12a3 has been detected in the lens cortex, core and fiber cells of rats (3). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: S5329-13B
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: Western Blot
Size: 100ul
Form: Supplied as a liquid in 0.1% BSA, 1mM sodium azide, 50% glycerol.
P type: Pab
Isotype: IgG
Purity: Serum
References: 1. Vallon V., Schroth J, Lang F, Kuhl D and Uchida S. (2009) Am J Physiol Renal Physiol. 297(3): F704-712. 2. Tiwari S., et al. (2009) Am J Nephrol. 30(6): 554-562. 3. Chee K.N., Vorontsova I., Lim J.C., Kistler J. and Donaldson P.J. (2010) Mol Vis. 16:800-812.
Additional info: Recognizes rat Slc12a3. Species Crossreactivity: Mouse, human

Get Quote

  • Best Price Guaranteed
  • Quick Response Time
  • Exclusive Promotions
Enquiry_down_arrow
United States Biological
Get a Quote Direct from
United States Biological

By submitting this form you agree to your details being passed to United States Biological for the purpose of generating the best quote*
See Full Product Listings >
Button_on Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave