Slc12a3, a thiazide-sensitive NaCl co-transporter, is found on the apical membrane of the distal convoluted tubule, where it is the principal mediator of Na+ and Clreabsorption in this segment of the nephron. It is activated by phosphorylation, and has been implicated in renal NaCl and K+ homeostasis (1). Regulation of Slc12a3 expression and phosphorylation by dietary NACl restriction appears to involve SGK1(1). In experiments with angiotensin II-infused mice, increased sensitivity to Ang II may involved over-activity of Slc12a3 (2). Therefore, Slc12a3 is the target of thiazide diuretics used in the treatment of hypertension (1). Molecular experiments have also shown that Slc12a3 has been detected in the lens cortex, core and fiber cells of rats (3).
Suitable for use in Western Blot. Other applications not tested.
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.